Journal:

Article Title: Identification and Characterization of CD44RC, a Novel Alternatively Spliced Soluble CD44 Isoform that can Potentiate the Hyaluronan Binding Activity of Cell Surface CD44

doi:

Figure Lengend Snippet: Expression of CD44RC in K562.CD44RC. The expression of CD44RC in vector alone control K562 cells (pCEP4) and K562 cells transfected with pCEP4.CD44RC (CD44RC) was determined by RT-PCR. Two primer pairs were used: (a) full-length CD44 cDNAs were amplified using primers specific for exons 1 (5′ CD44) and 20 (3′ CD44); and (b) CD44RC transcripts were specifically amplified using primers specific for exon 1 (5′ CD44) and the unique junction generated between exons 2 and 18 (RC unique) found only in CD44RC.

Article Snippet: Cell Lines The erytholeukemic cell line K562, the histiocytic cell line U937, the promyelocytic cell line HL60, and the myelomonocytic cell line KG1 and its less mature derivative KG1a, were all obtained from the American Type Culture Collection (ATCC; Rockville, MD).

Techniques: Expressing, Plasmid Preparation, Control, Transfection, Reverse Transcription Polymerase Chain Reaction, Amplification, Generated

Journal:

Article Title: Identification and Characterization of CD44RC, a Novel Alternatively Spliced Soluble CD44 Isoform that can Potentiate the Hyaluronan Binding Activity of Cell Surface CD44

doi:

Figure Lengend Snippet: Effect of CD44RC on cellular adhesion to hyaluronan. KG1 and KG1a cells were incubated with tissue culture medium or with anti-CD44 mAb 3G12 tissue culture supernatant for 1 hour at 4°C and washed prior to treatment with control tissue culture medium (NIL) or media conditioned by K562.CD44RC or K562.pCEP4 as described in Materials and Methods section. Following extensive washing, cells were assayed for their ability to adhere to hyaluronan-coated plastic. Each point represents the mean±SD of at least three independent determinations.

Article Snippet: Cell Lines The erytholeukemic cell line K562, the histiocytic cell line U937, the promyelocytic cell line HL60, and the myelomonocytic cell line KG1 and its less mature derivative KG1a, were all obtained from the American Type Culture Collection (ATCC; Rockville, MD).

Techniques: Incubation, Control

Journal:

Article Title: Identification and Characterization of CD44RC, a Novel Alternatively Spliced Soluble CD44 Isoform that can Potentiate the Hyaluronan Binding Activity of Cell Surface CD44

doi:

Figure Lengend Snippet: Effect of chondroitinase treatment on cellular adhesion to hyaluronan induced by CD44RC. KG1 cells were pretreated for 1 hour at 37°C with control tissue culture medium (NIL), chondroitinase ABC (CS'ase), or hyaluronan lyase (HA'ase) prior to incubation with K562.pCEP4 or K562.CD44RC conditioned tissue culture supernatants as described in Materials and Methods section. Treated cells were washed extensively and then assayed for their ability to adhere to hyaluronan-coated plastic. Each point represents the mean±SD of at least three independent determinations.

Article Snippet: Cell Lines The erytholeukemic cell line K562, the histiocytic cell line U937, the promyelocytic cell line HL60, and the myelomonocytic cell line KG1 and its less mature derivative KG1a, were all obtained from the American Type Culture Collection (ATCC; Rockville, MD).

Techniques: Control, Incubation

Journal: Molecular biology reports

Article Title: Dishevelled proteins and CYLD reciprocally regulate each other in CML cell lines.

doi: 10.1007/s11033-017-4122-3

Figure Lengend Snippet: Fig. 1 K562, MEG01and KU812 cell lines express CYLD mRNA. CYLD mRNA expression was assessed in three different CML cell lines. PCR results showing CYLD (1 K562, 2 MEG01, 3 Ku812) and β-actin (4 K562, 5 MEG01, 6 KU812) gene expression on 1% aga- rose gel. β-actin was used as loading control (bands; CYLD: 627 bp, β-actin: 318 bp; M-Marker: O’ Range Ruler 1 kb DNA ladder- Thermo Scientific)

Article Snippet: BCR/ABL positive cell lines K562, MEG01 and KU812 were purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH).

Techniques: Expressing, Gene Expression, Control, Marker

Journal: Frontiers in Immunology

Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

doi: 10.3389/fimmu.2020.00055

Figure Lengend Snippet: Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3, and KYAT4 in glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma cells (HNSCC). The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM ( N = 13, HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines [ N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC14, UT-SCC-15 ].

Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

doi: 10.3389/fimmu.2020.00055

Figure Lengend Snippet: IDO1 immunohistochemistry. Representative images of primary glioblastoma multiforme (GBM) [HROG36 (a,b) , HROG63 (c,d) , and HROG73 (e,f) ] and head and neck squamous cell carcinoma (HNSCC) [HNSCC06 (g,h) , HNSCC02 (i,j) , HNSCC01 (k,l) ] samples. Left panel: Routine HE staining. Right panel: Note the focal IDO1 expression on tumor-infiltrating lymphocytes exclusively in HNSCC cases. HNSCC case 1 (g,h) : tonsil (HPV positive ); case 2 (i,j) : mouth base (HPV negative , relapse); case 3 (k,l) : larynx (HPV negative ). Pictures were taken at 20× and 10× magnification, respectively.

Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Frontiers in Immunology

Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

doi: 10.3389/fimmu.2020.00055

Figure Lengend Snippet: Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3 , and KYAT4 as well as IDO1 protein abundance following interferon-γ (IFNγ) stimulation in glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma (HNSCC) cells. The cell cultures were either untreated or treated with IFNγ (50 ng/ml) for 24 h. (A) The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM N = 5 cell lines (HROG02, HROG05, HROG52, HROG63, HROG75); HNSCC, N = 4 cell lines (FADU, Detroit-562, Cal-33, PE/CA/PJ-15). (B) IDO1 immunofluorescence in selected cell lines. Cell nuclei were stained with DAPI, and IDO1 was depicted by monoclonal rabbit IDO1 primary antibody (1:100; Cell Signaling Technology), followed by secondary antibody (1:250, Boster Biological Technology Pleasanton, CA, United States) labeling. Cells were analyzed with a Zeiss LSM-780 Confocal Laser Microscope. Original magnification 20×.

Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

Techniques: Expressing, Quantitative Proteomics, Immunofluorescence, Staining, Labeling, Microscopy

Journal: Frontiers in Immunology

Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

doi: 10.3389/fimmu.2020.00055

Figure Lengend Snippet: Relative messenger RNA (mRNA) expression of IDO1, KYAT1, KYAT2, KYAT3, KYAT4 , and KMO upon cytostatic drugs and targeted therapy in head and neck squamous cell carcinoma (HNSCC) cells. Graphs indicate the mRNA expression of selected KP-related genes normalized to the housekeeping genes (2 −Δ CT). Results show data of three independent experiments using HNSCC cell line Detroit-562. * p < 0.05; ** p < 0.01. One-way ANOVA (Bonferroni's multiple comparison test).

Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

Techniques: Expressing, Comparison

Journal: Frontiers in Immunology

Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

doi: 10.3389/fimmu.2020.00055

Figure Lengend Snippet: Indoleamine 2,3-dioxygenase (IDO1) protein abundance in selected glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma (HNSCC) cells upon cytostatic drugs and targeted therapy. The cell cultures [ (A) GBM: HROG05; (B) HNSCC: Detroit-562] were either left untreated or treated with IFNγ (50 ng/ml) for 24 h. Treatments were performed simultaneously, i.e., IFNγ ± TMZ, Cetuximab, and/or Dinaciclib. Cell nuclei were stained with DAPI. Original magnification 20×. (C,D) Quantification was done to score staining intensity in untreated and treated HROG05 and Detroit-562 cells. This was carried out using ImageJ software as described in Material and Methods.

Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

Techniques: Quantitative Proteomics, Staining, Software